Office Phone: 509-335-9413
Lab Phone: 509-335-7642
- BS (Biology/chemistry): Mount Olive College, North Carolina
- Ph.D. (Biochemistry): Medical College of Wisconsin, Wisconsin
- Post-doctoral (Virology): Cleveland Clinic, Ohio
Host defense mechanism; antiviral response and inflammation: innate immune antiviral and inflammatory response against respiratory RNA viruses like human respiratory syncytial virus (RSV), and influenza A virus (flu).
Respiratory RNA virus infection and inflammation
Respiratory viruses like RSV, flu cause severe lung disease that manifest as pneumonia and bronchiolitis. These two diseases develop due to massive inflammation in the lung. Therefore, we are investigating the mechanism by which RSV and flu induce inflammation in the lung during infection. Particularly, we are interested in identifying and characterizing cellular factor that regulate inflammation. Our lab is focused on elucidating the mechanism of inflammasome activation by viruses. Inflammasome is an intracellular multi-protein complex involved in initiating inflammation by virtue of controlling production of pro-inflammatory mediators and inducing cell death. Our lab is investigating the biochemical, cellular and molecular mechanism responsible for inflammasome complex formation and activation during infection. Our studies have potential to be developed as therapeutic targets to control inflammation (and associated diseases like pneumonia) during respiratory virus infection.
Innate immune antiviral response against respiratory RNA viruses
We are studying the innate immune response (the first line of defense against pathogens) against RSV and flu. These respiratory viruses are highly pathogenic and cause diseases (pneumonia, bronchiolitis) in children/infants and elderly. We are investigating the mechanism of host derived antiviral immune response to identify novel antiviral molecules/pathways, which could be potentially developed as effective antiviral therapy or used as an immunemodulating adjuvant during vaccination. Our studies on innate immune response constitutes investigation of the role of two signaling pathways, NF-kappa B and interferon-alpa/beta induced JAK/STAT pathways in establishing an antiviral state. The long-term goal of our research is to identify and characterize a) the molecules that play a critical role in activation of these pathways and, b) the antiviral factors that are induced by NF-kappa B and JAK/STAT signaling cascades.
In summary, our laboratory encompasses several aspects of cell and molecular biology/virology with emphasis on
a) innate immune antiviral signal transduction pathway,
b) cellular/molecular mechanism that triggers inflammation during virus infection, and
c) virus-host/cell interaction.
- Pokharel SM, Shil NK, Bose S. (2016) Autophagy, TGF-β, and SMAD-2/3 Signaling Regulates Interferon-β Response in Respiratory Syncytial Virus Infected Macrophages. Front. Cell. Infect. Microbiol. 6: 174. PMCID: PMC5149518
- Tsai S, Segovia JA, Chang T, Shil NK, Pokharel SM, Kannan TR, Baseman JB, Defrene J, Pagé N, Cesaro A, Tessier PA, Bose S. (2015) Regulation of TLR3 activation by S100A9. J. Immunol. 195:4426-4437.
- Segovia JA, Tsai S, Chang T, Shil NK, Weintraub ST, Short JD, Bose S (2015) Nedd8 regulates inflammasome-dependent caspase-1 activation. Mol. Cell. Biol. 35:582-597 PMID: 25452302 PMCID: PMC4285429
- Bose S, Segovia J, Somarajan SE, Chang T, Kannan TR, Baseman JB (2014) ADP-ribosylation of NLRP3 by Mycoplasma pneumoniae CARDS toxin regulates inflammasome activity. MBio. 5: pii: e02186-14 PMID: 25538194 PMCID: PMC4278538
- Tsai S, Segovia J, Mgbemena V, Chang T, Berton MT, Morris I, Tardiff M, Tessier P, Cesaro A, Bose S (2014) DAMP Molecule S100A9 Acts as a Molecular Pattern to Enhance Inflammation during Influenza A Virus Infection: Role of DDX21-TRIF-TLR4-MyD88 Pathway. PLoS. Pathogens. 10(1): e1003848 PMID: 24391503 PMCID: PMC3879357
- Thinwa J, Segovia JA, Bose S, Dube PH (2014) Integrin-mediated first signal for inflammasome activation in intestinal epithelial cells. J. Immunol. 193:1373-82 PMID: 24965773 PMCID: PMC4174679
- Mgbemena V, Segovia J, Chang T, Bose S (2013) KLF6 and iNOS regulates apoptosis during respiratory syncytial virus infection. Cellular Immunol. 283:1-7 PMID: 23831683 PMCID: PMC3744625
- Mgbemena V, Segovia J, Chang T, Tsai S-Y, Cole G. T, Hung C-Y, Bose S (2012) Transactivation of inducible nitric oxide synthase gene by Krüppel-like factor 6 regulates apoptosis during influenza A virus infections. J. Immunol. 189:606-615 PMID: 22711891 PMCID: PMC3392426
- Bose R, Thinwa J, Chaparro P, Zhong Y, Bose S, Zhong G, Dube PH (2012) Mitogen-activated protein kinase-dependent interleukin-1α intracrine signaling is modulated by YopP during Yersinia enterocolitica infection. Infect. Immun. 80(1):289-97. doi: 10.1128/IAI.05742-11. Epub 2011 Nov 14. PMID: 22083707 PMCID: PMC3255648
- Chang T, Segovia J.A, Sabbah A, Mgbemena V, Bose S (2012) Role of cholesterol-rich lipid raft in human respiratory syncytial virus infection. Virology 422: 205-211 PMID: 22088217 PMCID: PMC3249476
- Hinojosa C.A, Mgbemena V, Van Roekel S, Austad S. N, Miller R. A, Bose S, Orihuela C.J (2012) Enteric-delivered rapamycin enhances resistance of aged mice to pneumococcal pneumonia through reduced cellular senescence. Exp. Gerontol. 47:958-965 PMID: 22981852 PMCID: PMC3490008
- Segovia J, Sabbah A, Mgbemena V, Tsai SY, Chang TH, Berton MT, Morris IR, Allen IC, Ting JP, Bose S (2012) TLR2/MyD88/NF-kappa B pathway, reactive oxygen species, K+ efflux activates NLRPR3/ASC inflammasome during respiratory syncytial virus infection. PLoS. One. 7(1):e29695. doi: 10.1371/journal.pone.0029695. Epub 2012 Jan 25. PMID: 22295065 PMCID: PMC3266238
- Echchgadda I, Chang TH, Sabbah A, Bakri I, Ikeno Y, Hubbard GB, Chatterjee B, Bose S (2011) Oncolytic targeting of androgen-sensitive prostate tumor by the respiratory syncytial virus (RSV): consequences of deficient interferon-dependent antiviral defense. BMC. Cancer. 11:43. doi: 10.1186/1471-2407-11-43. PMID: 21276246 PMCID: PMC3038980
- Kota S, Echchgadda I, DeLa Cruz I, Sabbah A, Chang T, Harnack R, Mgbemena, Chatterjee B, Bose S (2009) Anti-cancer oncolytic activity of respiratory syncytial virus. Cancer. Gene. Ther. 16: 923-935 PMID: 19444304 PMCID: PMC2813688
- Sabbah A, Chang T, Harnack R, Frohlich V, Dube PH, Tominaga K, Xiang Y, Bose S (2009) Activation of innate immune antiviral response by Nod2. Nature. Immunol. 10(10):1073-80. doi: 10.1038/ni.1782. Epub 2009 Aug 23. PMID: 19701189 PMCID: PMC2752345
- Kota S, Sabbah A, Chang T. H, Harnack R, Xiang Y, Meng Y, Bose S (2008) Role of human beta-defensin-2 during tumor necrosis factor-alpha/NF-kappa B mediated innate anti-viral response against human respiratory syncytial virus. J. Biol. Chem. 283: 22417-22429
- Bose S, Basu M, Banerjee A. K. (2004) Role of nucleolin in human parainfluenza virus type 3 infection of human lung epithelial cells. J. Virol. 78: 8146-8158 PMID: 15254186 PMCID: PMC446124
- Dong B, Zhou Q, Zhao J, Zhou A, Harty R.N., Bose S, Banerjee A, Slee R, Guenther J, Williams B.R., Wiedmer T, Sims P.J., Silverman R.H. (2004) Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J. Virol. 78: 8983-8993 PMID: 15308695 PMCID: PMC506946
- Bose S, Kar N, Maitra R, DiDonato JA, Banerjee AK (2003) Temporal activation of NF-kappaB regulates an interferon-independent innate antiviral response against cytoplasmic RNA viruses. Proc. Natl. Acad. Sci. USA. 100(19):10890-5. Epub 2003 Sep 5. PMID: 12960395 PMCID: PMC196898
- Bose S, Malur A, Banerjee AK (2001) Polarity of human parainfluenza virus type 3 infection in polarized human lung epithelial A549 cells : Role of microfilament and microtubule.
J. Virol. 75 : 1984-1989 PMID: 11160698 PMCID: PMC115145