The T2Bo Genome sequence
The Texas T2Bo strain was selected for genomic sequencing because the history of this isolate is well documented: it is known to be virulent, and it is tick transmissible. A combined clone by clone and whole genome shotgun approach was used to obtain the complete genome sequence for this organism. The resulting sequence contains 9 contigs which may be downloaded by following the links below.
Other B. bovis strains
The completed T2Bo sequence can now be used to scaffold genome assemblies of other Babesia bovis isolates that are generated with next generation sequencing technologies. We are generating paired sequences for virulent B. bovis strains and their attenuated counterparts, generated by rapid, successive inoculation in cattle.
B. bovis genomics papers
Brayton, K. A., A. O.T. Lau, D. R. Herndon, L. Hannick, L. S. Kappmeyer, S. J. Berens, S. L. Bidwell, W. C. Brown, J. Crabtree, D. Fadrosh, T. Feldblum, H. A. Forberger, B. J. Haas, J. M. Howell, H. Khouri, H. Koo, D. J. Mann, J. Norimine, I. T. Paulsen, D. Radune, Q. Ren, R. K. Smith Jr., C. E. Suarez, O. White, J. R. Wortman, D. P. Knowles Jr, T. F. McElwain, and V. M. Nene. Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoa. PLOS Pathogens. 3: 1401-1413. 2007.
Roalson, E. H., T. F. McElwain, K. A. Brayton, D. P. Knowles, and A. O. T. Lau. Babesia bovis: a comprehensive phylogenetic analysis of plastid-encoded genes supports green algal origin of apicoplasts. Experimental Parasitology. 123: 236-243. 2009.
Mesplet M., G. H. Palmer, M. J. Pedroni, I. Echaide, M. Florin-Christensen, L. Schnittger, and A. O. Lau. Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair. Mol Biochem Parasitol. 179: 111-113. 2011.
Lau, A. O., A. Kalyanaraman, I. Echaide, G. H. Palmer, R. Bock, M. J. Pedroni, M. Rameshkumar, M. B. Ferreira, T. I. Fletcher, and T. F. McElwain. Attenuation of virulence in an apicomplexan hemoparasite results in reduced genome diversity at the population level. BMC Genomics. 12: 410. 2011.
Ferreri, L. M., K. A. Brayton, K. S. Sondgeroth, A. O. T. Lau, C. E. Suarez, and T. F. McElwain. Expression and strain variation of the novel “Small Open Reading Frame” (smorf) multigene family in Babesia bovis. International Journal for Parasitology. 42: 131-138. 2012.
Pedroni, M. J., K. S. Sondgeroth, G. M.,Gallego-Lopez I. Echaide, and A.O. Lau. Comparative transcriptome analysis of geographically distinct virulent and attenuated Babesia bovis strains reveals similar gene expression changes through attenuation. BMC Genomics. 14: 763. 2013.
BLAST Babesia contigs
BLAST on local server
Theileria equi Sequencing Projects
Theileria equi has been named Piroplasma equi, Nuttallia equi, and Babesia equi. T. equi causes equine piroplasmosis, a disease that results in restricted movement of horses worldwide. The disease occurs throughout the tropical and subtropical areas of the world, with endemic areas in many parts of Europe, Asia, Arabia, South and Central America and Africa. T. equi is transmitted by species of ixodid ticks of the genera Dermacentor, Rhipicephalus and Hyalomma. The Florida isolate, also known as the USDA strain, was sequenced in collaboration with the J. Craig Venter Institute (JCVI) using a whole genome shotgun approach. The resulting sequence contains 10 contigs as pictured below. Please note the order of the internal contigs of chromosome 4 are not known.
T. equi genomics papers
Kappmeyer, L. S., M. Thiagarajan, D. R. Herndon, J. D. Ramsay, E. Caler, A Djikeng, J. J. Gillespie, A. O. T. Lau, E. H. Roalson, J. C. Silva, M. G. Silva, C. E. Suarez, M. W. Ueti, V. M. Nene, R. H. Mealey, D P. Knowles and K. A. Brayton. Comparative genomic analysis and phylogenetic position of Theileria equi. BMC Genomics. 13: 603. 2012.
Bastos, R. G., C. E. Suarez, J. M. Laughery, W. C. Johnson, M. W. Ueti, and D. P. Knowles. Differential expression of three members of the multidomain adhesion CCp family in Babesia bigemina, Babesia bovis and Theileria equi. PLoS One. 8: e67765. 2013.
These projects are funded by the USDA Agricultural Research Service - Animal Disease Research Unit (ARS-ADRU), USDA Formula Funds, The Wellcome Trust and the CVM Animal Health Research Center.
Please contact Audrey Lau