First Wash Buffer (FWB)
450 ml PBS
50 ml ACD
2.25 ml 20% sodium azide in PBS
10 ml horse serum
Second Wash Buffer (SWB) FWB without horse serum
eBiosciences Foxp3 Buffer Kit (Cat. No. 00-5523-00)
10X Permeabilization buffer
Perm/Wash solution: dilute 10X Permeabilization buffer 1:10 with distilled water. Make up just before use.
Fix/Perm solution: Dilute 1 part of Fixation/Permeabilization concentrate with 3 parts of Fixation/Permeabilization diluent. Make up just before use.