Use of Propidium Iodide for Labeling Dead Cells for Flow Cytometry
- Label cells with primary and secondary antibodies using the standard procedure and FITC conjugates.
- After the final wash, resuspend the cells in 200 μl of a 1:1000 dilution of PI stock. Stock PI is 0.5 mg/ml in PBS. Dilute 10 ml stock in 10 μl PBS before using. Analyze on flow cytometer immediately.
- The dead cells will be labeled with PI and will show up in FL3.