College of Veterinary Medicine

Veterinary Microbiology and Pathology

Use of Propidium Iodide for Labeling Dead Cells for Flow Cytometry



  1. Label cells with primary and secondary antibodies using the standard procedure and FITC conjugates.
  2. After the final wash, resuspend the cells in 200 μl of a 1:1000 dilution of PI stock. Stock PI is 0.5 mg/ml in PBS. Dilute 10 ml stock in 10 μl PBS before using. Analyze on flow cytometer immediately.
  3. The dead cells will be labeled with PI and will show up in FL3.
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